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ASCP ASCP-MLT Exam Syllabus Topics:

SectionWeightObjectives
Topic 1: Hematology20–25%- Physiology and disease states
- Laboratory testing and hemostasis
Topic 2: Microbiology15–20%- Bacteriology and mycology procedures
- Virology and parasitology testing
Topic 3: Immunology5–10%- Serologic procedures and immune diseases
- Principles of immunology
Topic 4: Laboratory Operations5–10%- Quality assessment and safety
- Instrumentation and laboratory mathematics
Topic 5: Blood Banking15–20%- Transfusion practice and testing
- Blood group systems and immunology
- Blood products and components
Topic 6: Urinalysis and Other Body Fluids5–10%- Microscopic analysis and disease correlation
- Physical and chemical testing
Topic 7: Chemistry20–25%- Carbohydrates, lipids, and enzymes
- Electrolytes, acid-base, and toxicology

ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:

1. CAMP is the correct answer because the case is typical of Streptococcus agalactiae(Group B strep). The main presumptive identification test is CAMP. Bile solubility is positive for Streptococcus pneumonia. Coagulase would be positive for Staphylococcus aureus. PYR is positive for Streptococcus pyogenes (Group A strep) which is ruled out by the resistance to bacitracin.
Microbiology
Spinal fluid cultures on a 3-day-old infant revealed beta-hemolytic, Gram-positive cocci occurring in pairs and chains. The organism was catalase-negative and bile esculin-negative. Resistance to 0.04 U disk of bacitracin was noted. The other test necessary to identify this organism is a positive test by:

A) CAMP
B) Coagulase
C) Bile solubility
D) PYR


2. Kell, Kidd, and Duffy are usually warm reacting IgG antibodies. The most common P system antibody is anti-P1, which is a frequently naturally occurring low titer IgM antibody, seen in most P2 individuals.
Blood bank
Which of the following blood groups is most frequently associated with cold agglutinins:

A) Kell
B) Duffy
C) Kidd
D) P


3. This drawing depicts beta thalassemia minor B+/B. In Beta thalassemia minor B+/B, one beta gene locus is partially deleted or inactive.

Hematology
This drawing depicts which beta chain genotype ?

A) Beta thalassemia minor
B) Beta thalassemia intermedia
C) Delta-beta thalassemia minor
D) Beta thalassemia major


4. Where can one find guidance on the minimum performance standards for clinical laboratories?

A) Food and Drug Administration (FDA)
B) National Institutes of Health
C) Clinical Laboratory Improvement Amendments of 1988 (CLIA '88)
D) Centers for Disease Control and Prevention (CDC)


5. Gluconeogenesis is the formation of glucose from noncarbohydrates when carbohydrate intake is absent, a fasting state. The hormone cortisol along with glucagon and epinephrine all stimulate this metabolic pathway.
Insulin; however, inhibits this pathway and is therefore the correct answer.
Which of the following hormones inhibits gluconeogenesis, the formation of glucose from noncarbohydrate sources such as amino acids, glycerol, and fatty acids?

A) Cortisol
B) Insulin
C) Glucagon
D) Epinephrine


Solutions:

Question # 1
Answer: A
Question # 2
Answer: D
Question # 3
Answer: A
Question # 4
Answer: C
Question # 5
Answer: B

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